Document Details

Document Type : Article In Journal 
Document Title :
Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug‐Resistant CTX‐M‐Producing Bacterial Pathogens
Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug‐Resistant CTX‐M‐Producing Bacterial Pathogens
 
Document Language : English 
Abstract : Multidrug-resistance due to “b lactamases having the expanded spectrum” (ESBLs) in members of Enterobacteriaceae is a matter of continued clinical concern. CTX-M is among the most common ESBLs in Enterobacteriaceae family. In the present study, a nanoformulation of cefotaxime was prepared using gold nanoparticles to combat drug-resistance in ESBL producing strains. Here, two CTX-M-15 positive cefotaxime resistant bacterial strains (i.e., one Escherichia coli and one Klebsiella pneumoniae strain) were used for testing the efficacy of “cefotaxime loaded goldnanoparticles.” Bromelain was used for both reduction and capping in the process of synthesis of gold-nanoparticles. Thereafter, cefotaxime was conjugated onto it with the help of activator 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide. For characterization of both unconjugated and cefotaxime conjugated gold nanoparticles; UV-Visible spectroscopy, Scanning, and Transmission type Electron Microscopy methods accompanied withDynamic Light Scattering were used.We used agar diffusion method plus microbroth-dilutionmethod for the estimation of the antibacterial-activity and determination ofminimuminhibitory concentration or MIC values, respectively.MIC values of cefotaxime loaded gold nanoparticles against E. coli and K. pneumoniae were obtained as 1.009 and 2.018 mg/L, respectively. These bacterial strains were completely resistant to cefotaxime alone. These results reinforce the utility of conjugating an old unresponsive antibiotic with gold nanoparticles to restore its efficacy against otherwise resistant bacterial pathogens. 
ISSN : 1097-4644 
Journal Name : Journal of Cellular Biochemistry 
Volume : 1 
Issue Number : 1 
Publishing Year : 1438 AH
2017 AD 
Article Type : Article 
Added Date : Sunday, May 28, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Shaikh SibhghatullaSibhghatulla, Shaikh InvestigatorDoctorate 
Rizvi Syed MohdSyed Mohd, Rizvi ResearcherDoctorate 
Shakil ShaziShazi, Shakil ResearcherDoctorate 
Hussain TalibTalib, Hussain ResearcherDoctorate 
Alshammari Thamir MThamir M, Alshammari ResearcherDoctorate 
Ahmad WaseemWaseem, Ahmad ResearcherDoctorate 
Tabrez ShamsShams, Tabrez ResearcherDoctorate 
Al‐Qahtani Mohammad HMohammad H, Al‐Qahtani ResearcherDoctorate 
Abuzenadah Adel MAdel M, Abuzenadah ResearcherDoctorate 

Files

File NameTypeDescription
 40789.pdf pdf 

Back To Researches Page